Cis- and cell-type-dependent trans-requirements for Lassa virus-like particle production.
J Gen Virol
; 96(Pt 7): 1626-35, 2015 Jul.
Article
en En
| MEDLINE
| ID: mdl-25722347
ABSTRACT
Lassa virus (LASV) small zinc-finger protein (Z), which contains two L-domain motifs, plays a central role in virus budding. Here, we report that co-expression of glycoprotein (GPC) altered the requirements for cholesterol but not the L-domains and host factor, Tsg101, for Z-induced virus-like particle (VLP) production. In particular, the cholesterol requirement for VLP production was cell-type-dependent. In addition, GPC was found to be important for co-localization of Z with CD63, a late endosomal marker. We also found that the N-terminal region (aa 3-10) of Z was critical for its myristoylation and VLP production. These findings will contribute to our understanding of LASV assembly and budding.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Virales
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Ensamble de Virus
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Virosomas
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Liberación del Virus
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Virus Lassa
Límite:
Animals
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Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article