Identification of novel long noncoding RNAs underlying vertebrate cardiovascular development.
Circulation
; 131(14): 1278-1290, 2015 Apr 07.
Article
en En
| MEDLINE
| ID: mdl-25739401
ABSTRACT
BACKGROUND:
Long noncoding RNAs (lncRNAs) have emerged as critical epigenetic regulators with important functions in development and disease. Here, we sought to identify and functionally characterize novel lncRNAs critical for vertebrate development. METHODS ANDRESULTS:
By relying on human pluripotent stem cell differentiation models, we investigated lncRNAs differentially regulated at key steps during human cardiovascular development with a special focus on vascular endothelial cells. RNA sequencing led to the generation of large data sets that serve as a gene expression roadmap highlighting gene expression changes during human pluripotent cell differentiation. Stage-specific analyses led to the identification of 3 previously uncharacterized lncRNAs, TERMINATOR, ALIEN, and PUNISHER, specifically expressed in undifferentiated pluripotent stem cells, cardiovascular progenitors, and differentiated endothelial cells, respectively. Functional characterization, including localization studies, dynamic expression analyses, epigenetic modification monitoring, and knockdown experiments in lower vertebrates, as well as murine embryos and human cells, confirmed a critical role for each lncRNA specific for each analyzed developmental stage.CONCLUSIONS:
We have identified and functionally characterized 3 novel lncRNAs involved in vertebrate and human cardiovascular development, and we provide a comprehensive transcriptomic roadmap that sheds new light on the molecular mechanisms underlying human embryonic development, mesodermal commitment, and cardiovascular specification.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Vertebrados
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Sistema Cardiovascular
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Regulación del Desarrollo de la Expresión Génica
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Miocitos Cardíacos
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Células Madre Pluripotentes
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Células Endoteliales
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ARN Largo no Codificante
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article