Polymorphisms in the AOX2 gene are associated with the rooting ability of olive cuttings.
Plant Cell Rep
; 34(7): 1151-64, 2015 Jul.
Article
en En
| MEDLINE
| ID: mdl-25749737
ABSTRACT
KEY MESSAGE Different rooting ability candidate genes were tested on an olive cross progeny. Our results demonstrated that only the AOX2 gene was strongly induced. OeAOX2 was fully characterised and correlated to phenotypical traits. The formation of adventitious roots is a key step in the vegetative propagation of trees crop species, and this ability is under strict genetic control. While numerous studies have been carried out to identify genes controlling adventitious root formation, only a few loci have been characterised. In this work, candidate genes that were putatively involved in rooting ability were identified in olive (Olea europaea L.) by similarity with orthologs identified in other plant species. The mRNA levels of these genes were analysed by real-time PCR during root induction in high- (HR) and low-rooting (LR) individuals. Interestingly, alternative oxidase 2 (AOX2), which was previously reported to be a functional marker for rooting in olive cuttings, showed a strong induction in HR individuals. From the OeAOX2 full-length gene, alleles and effective polymorphisms were distinguished and analysed in the cross progeny, which were segregated based on rooting. The results revealed a possible correlation between two single nucleotide polymorphisms of OeAOX2 gene and rooting ability.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Oxidorreductasas
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Proteínas de Plantas
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Genes de Plantas
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Raíces de Plantas
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Polimorfismo de Nucleótido Simple
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Proteínas Mitocondriales
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Olea
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Año:
2015
Tipo del documento:
Article