Enzyme responsive drug delivery system based on mesoporous silica nanoparticles for tumor therapy in vivo.
Nanotechnology
; 26(14): 145102, 2015 Apr 10.
Article
en En
| MEDLINE
| ID: mdl-25789511
ABSTRACT
To reduce the toxic side effects of traditional chemotherapeutics in vivo, we designed and constructed a biocompatible, matrix metalloproteinases (MMPs) responsive drug delivery system based on mesoporous silica nanoparticles (MSNs). MMPs substrate peptide containing PLGLAR (sensitive to MMPs) was immobilized onto the surfaces of amino-functionalized MSNs via an amidation reaction, serving as MMPs sensitive intermediate linker. Bovine serum albumin was then covalently coupled to linker as end-cap for sealing the mesopores of MSNs. Lactobionic acid was further conjugated to the system as targeting motif. Doxorubicin hydrochloride was used as the model anticancer drug in this study. A series of characterizations revealed that the system was successfully constructed. The peptide-functionalized MSNs system demonstrated relatively high sensitivity to MMPs for triggering drug delivery, which was potentially important for tumor therapy since the tumor's microenvironment overexpressed MMPs in nature. The in vivo experiments proved that the system could efficiently inhibit the tumor growth with minimal side effects. This study provides an approach for the development of the next generation of nanotherapeutics toward efficient cancer treatment.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Portadores de Fármacos
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Doxorrubicina
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Sistemas de Liberación de Medicamentos
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Dióxido de Silicio
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Metaloproteinasas de la Matriz
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Nanopartículas
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Antineoplásicos
Límite:
Animals
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Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article