Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors.
Nat Chem Biol
; 11(5): 332-8, 2015 May.
Article
en En
| MEDLINE
| ID: mdl-25799144
ABSTRACT
Methionine can be reversibly oxidized to methionine sulfoxide (MetO) under physiological and pathophysiological conditions, but its use as a redox marker suffers from the lack of tools to detect and quantify MetO within cells. In this work, we created a pair of complementary stereospecific genetically encoded mechanism-based ratiometric fluorescent sensors of MetO by inserting a circularly permuted yellow fluorescent protein between yeast methionine sulfoxide reductases and thioredoxins. The two sensors, respectively named MetSOx and MetROx for their ability to detect S and R forms of MetO, were used for targeted analysis of protein oxidation, regulation and repair as well as for monitoring MetO in bacterial and mammalian cells, analyzing compartment-specific changes in MetO and examining responses to physiological stimuli.
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1
Banco de datos:
MEDLINE
Asunto principal:
Metionina
Límite:
Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article