Rapid diagnostic tests apply for pediatric infections at outpatient clinic setting.
Clin Lab
; 61(1-2): 195-9, 2015.
Article
en En
| MEDLINE
| ID: mdl-25807657
ABSTRACT
BACKGROUND:
Early identification of the etiology of infection is beneficial. Most infections are treated as outpatients. However, facilities for rapid diagnosis are not available in clinic settings.METHODS:
We applied Immunochromatography (IC) and Loop-mediated Isothermal Amplification (LAMP) methods to rapidly diagnose pathogens among 31 children with respiratory infection and 12 with gastroenteritis at a clinic in Saitama prefecture, Japan. Pathogens were then screened by multiplex conventional and real-time PCRs and bacterial culture.RESULTS:
Respiratory pathogens were found in 64.5%. Despite the narrow spectrum, rapid tests identified pathogens in 28.6% of cases with a high agreement rate of 89.3% with PCR. Gastroenteritis pathogens were found in 66.7%. E. coli was positive in 3 cases and all were negative for verotoxin by LAMP. The agreement rate of IC and PCR assay was high, 100%.CONCLUSIONS:
IC and LAMP are reliable and suitable methods in limited-resource settings for early pathogenic identification, which will help appropriate management, avoid unnecessary intervention, and cost saving.
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Banco de datos:
MEDLINE
Asunto principal:
Infecciones del Sistema Respiratorio
/
Sistemas de Atención de Punto
/
Enteritis
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Child
/
Child, preschool
/
Humans
/
Infant
Idioma:
En
Año:
2015
Tipo del documento:
Article