The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias.
Oncogene
; 4(12): 1449-56, 1989 Dec.
Article
en En
| MEDLINE
| ID: mdl-2594367
ABSTRACT
We have shown previously that spleen focus forming virus integration near the Spi-1 putative oncogene is observed in 95% of erythroid Friend tumors (Moreau-Gachelin et al., 1988). Here we describe how the proviral insertion in the Spi-1 domain is associated with the enhanced transcription of a 1.4 kb mRNA normally expressed at a low level in normal cells. The gene is localized on murine chromosome 2, band E3. The structure of the Spi-1 gene was determined by sequencing genomic and cDNA clones. The gene has an open reading frame encoding a protein of 218 amino acids, extending over five exons. Proviruses always integrate outside, upstream and in the opposite orientation of the protein-encoding domain. This suggests that SFFV integration activates the expression of Spi-2 gene that may contribute to the erythroleukemic process.
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Banco de datos:
MEDLINE
Asunto principal:
Oncogenes
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Leucemia Eritroblástica Aguda
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Proteínas Oncogénicas de Retroviridae
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Activación Transcripcional
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Mapeo Cromosómico
/
Proteínas de Unión al ADN
Límite:
Animals
Idioma:
En
Año:
1989
Tipo del documento:
Article