Novibacillus thermophilus gen. nov., sp. nov., a Gram-staining-negative and moderately thermophilic member of the family Thermoactinomycetaceae.
Int J Syst Evol Microbiol
; 65(8): 2591-2597, 2015 Aug.
Article
en En
| MEDLINE
| ID: mdl-25951858
ABSTRACT
Two Gram-staining-negative, facultatively anaerobic bacterial strains, SG-1T and SG-2, were isolated from a saline soil sample and a compost sample, respectively. The cells were non-motile rods that occurred singly or in chains, and endospores were not observed under tested growth conditions. Optimum growth occurred at 50 °C, pH 7.5-8.0 and with 5-7â% (w/v) NaCl. The DNA G+C content was 49.5-50.5âmol%. The strains contained MK-7 as the predominant menaquinone and iso-C15 0 and anteiso-C15 0 as the major fatty acids. The polar lipids consisted mainly of diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan type was A1γ (meso-DAP direct). Phylogenetic analyses revealed that the new isolates belonged to the family Thermoactinomycetaceae, exhibiting low 16S rRNA gene sequence similarity (90.8-91.3â%) to the nearest type strain, Mechercharimyces asporophorigenens YM11-542T, and formed a well-supported lineage that was clearly distinguished from all currently described genera in this family. Based on our polyphasic taxonomic characterization, we propose that strains SG-1T and SG-2 represent a novel genus and species within the family Thermoactinomycetaceae, for which we propose the name Novibacillus thermophilus gen. nov., sp. nov. The type strain of Novibacillus thermophilus is SG-1T ( = KCTC 33118T = CGMCC 1.12771T).
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Banco de datos:
MEDLINE
Asunto principal:
Filogenia
/
Microbiología del Suelo
/
Bacillales
País como asunto:
Asia
Idioma:
En
Año:
2015
Tipo del documento:
Article