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Development of a Custom-Designed, Pan Genomic DNA Microarray to Characterize Strain-Level Diversity among Cronobacter spp.
Tall, Ben Davies; Gangiredla, Jayanthi; Gopinath, Gopal R; Yan, Qiongqiong; Chase, Hannah R; Lee, Boram; Hwang, Seongeun; Trach, Larisa; Park, Eunbi; Yoo, YeonJoo; Chung, TaeJung; Jackson, Scott A; Patel, Isha R; Sathyamoorthy, Venugopal; Pava-Ripoll, Monica; Kotewicz, Michael L; Carter, Laurenda; Iversen, Carol; Pagotto, Franco; Stephan, Roger; Lehner, Angelika; Fanning, Séamus; Grim, Christopher J.
  • Tall BD; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Gangiredla J; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Gopinath GR; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Yan Q; UCD Centre for Food Safety, School of Public Health, Physiotherapy and Population Science, University College Dublin , Dublin , Ireland ; WHO Collaborating Centre for Cronobacter , Dublin , Ireland.
  • Chase HR; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Lee B; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Hwang S; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Trach L; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Park E; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Yoo Y; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Chung T; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Jackson SA; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Patel IR; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Sathyamoorthy V; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Pava-Ripoll M; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , College Park, MD , USA.
  • Kotewicz ML; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Carter L; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
  • Iversen C; College of Life Sciences, University of Dundee , Dundee , UK.
  • Pagotto F; Food Directorate, Bureau of Microbial Hazards/Health Canada , Ottawa, ON , Canada.
  • Stephan R; Institute for Food Safety and Hygiene, University of Zurich , Zurich , Switzerland.
  • Lehner A; Institute for Food Safety and Hygiene, University of Zurich , Zurich , Switzerland.
  • Fanning S; UCD Centre for Food Safety, School of Public Health, Physiotherapy and Population Science, University College Dublin , Dublin , Ireland ; WHO Collaborating Centre for Cronobacter , Dublin , Ireland.
  • Grim CJ; Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.
Front Pediatr ; 3: 36, 2015.
Article en En | MEDLINE | ID: mdl-25984509
ABSTRACT
Cronobacter species cause infections in all age groups; however neonates are at highest risk and remain the most susceptible age group for life-threatening invasive disease. The genus contains seven speciesCronobacter sakazakii, Cronobacter malonaticus, Cronobacter turicensis, Cronobacter muytjensii, Cronobacter dublinensis, Cronobacter universalis, and Cronobacter condimenti. Despite an abundance of published genomes of these species, genomics-based epidemiology of the genus is not well established. The gene content of a diverse group of 126 unique Cronobacter and taxonomically related isolates was determined using a pan genomic-based DNA microarray as a genotyping tool and as a means to identify outbreak isolates for food safety, environmental, and clinical surveillance purposes. The microarray constitutes 19,287 independent genes representing 15 Cronobacter genomes and 18 plasmids and 2,371 virulence factor genes of phylogenetically related Gram-negative bacteria. The Cronobacter microarray was able to distinguish the seven Cronobacter species from one another and from non-Cronobacter species; and within each species, strains grouped into distinct clusters based on their genomic diversity. These results also support the phylogenic divergence of the genus and clearly highlight the genomic diversity among each member of the genus. The current study establishes a powerful platform for further genomics research of this diverse genus, an important prerequisite toward the development of future countermeasures against this foodborne pathogen in the food safety and clinical arenas.
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Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Año: 2015 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Año: 2015 Tipo del documento: Article