[Affinity modification of DNA-dependent RNA-polymerase from phage T7 with 5'-p-fluorosulfonylbenzoyl adenosine: the effect of modification on the interaction with substrates]. / Affinnaia modifikatsiia DNK-zavisimoi RNK-polimerazy faga T7 5'-p-ftorsul'fonilbenzoiladenozinom: vliianie modifikatsii na vzaimodeistvie s substratami.
Mol Biol (Mosk)
; 23(5): 1273-8, 1989.
Article
en Ru
| MEDLINE
| ID: mdl-2608036
ABSTRACT
T7 RNA polymerase, covalently modified with 5'-p-fluorosulfonylbenzoyl adenosine, looses the ability of binding the promoter (pGEM-2 plasmid) and poly(dC) template as well as the initiating nucleoside triphosphate (GTP). However the enzyme retains the unspecific binding with DNA fragments of considerable length.
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Banco de datos:
MEDLINE
Asunto principal:
Fagos T
/
ARN Polimerasas Dirigidas por ADN
/
Marcadores de Afinidad
/
Adenosina
Idioma:
Ru
Año:
1989
Tipo del documento:
Article