Mitochondrial ATP synthasome: Expression and structural interaction of its components.
Biochem Biophys Res Commun
; 464(3): 787-93, 2015 Aug 28.
Article
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| MEDLINE
| ID: mdl-26168732
ABSTRACT
Mitochondrial ATP synthase, ADP/ATP translocase (ANT), and inorganic phosphate carrier (PiC) are supposed to form a supercomplex called ATP synthasome. Our protein and transcript analysis of rat tissues indicates that the expression of ANT and PiC is transcriptionally controlled in accordance with the biogenesis of ATP synthase. In contrast, the content of ANT and PiC is increased in ATP synthase deficient patients' fibroblasts, likely due to a post-transcriptional adaptive mechanism. A structural analysis of rat heart mitochondria by immunoprecipitation, blue native/SDS electrophoresis, immunodetection and MS analysis revealed the presence of ATP synthasome. However, the majority of PiC and especially ANT did not associate with ATP synthase, suggesting that most of PiC, ANT and ATP synthase exist as separate entities.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Adenosina Trifosfato
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ATPasas de Translocación de Protón Mitocondriales
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Mitocondrias
Límite:
Animals
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Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article