Comprehensive analytical comparison of strategies used for small molecule aptamer evaluation.
Anal Chem
; 87(17): 8608-12, 2015 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-26192270
ABSTRACT
Nucleic acid aptamers are versatile molecular recognition agents that bind to their targets with high selectivity and affinity. The past few years have seen a dramatic increase in aptamer development and interest for diagnostic and therapeutic applications. As the applications for aptamers expand, the need for a more standardized, stringent, and informative characterization and validation methodology increases. Here we performed a comprehensive analysis of a panel of conventional affinity binding assays using a suite of aptamers for the small molecule target ochratoxin A (OTA). Our results highlight inconsistency between conventional affinity assays and the need for multiple characterization strategies. To mitigate some of the challenges revealed in our head-to-head comparison of aptamer binding assays, we further developed and evaluated a set of novel strategies that facilitate efficient screening and characterization of aptamers in solution. Finally, we provide a workflow that permits rapid and robust screening, characterization, and functional verification of aptamers thus improving their development and integration into novel applications.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Química Analítica
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Aptámeros de Nucleótidos
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Técnica SELEX de Producción de Aptámeros
Idioma:
En
Año:
2015
Tipo del documento:
Article