Imidase catalyzing desymmetric imide hydrolysis forming optically active 3-substituted glutaric acid monoamides for the synthesis of gamma-aminobutyric acid (GABA) analogs.

Nojiri, Masutoshi; Hibi, Makoto; Shizawa, Hiroaki; Horinouchi, Nobuyuki; Yasohara, Yoshihiko; Takahashi, Satomi; Ogawa, Jun

Nojiri, Masutoshi; Hibi, Makoto; Shizawa, Hiroaki; Horinouchi, Nobuyuki; Yasohara, Yoshihiko; Takahashi, Satomi; Ogawa, Jun.

Nojiri M; Biotechnology Development Laboratories, Kaneka Corporation, 1-8 Miyamae, Takasago, Hyogo, 676-8688, Japan.
Hibi M; Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
Shizawa H; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
Horinouchi N; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
Yasohara Y; Biotechnology Development Laboratories, Kaneka Corporation, 1-8 Miyamae, Takasago, Hyogo, 676-8688, Japan.
Takahashi S; Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
Ogawa J; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan. ogawa@kais.kyoto-u.ac.jp.

Appl Microbiol Biotechnol ; 99(23): 9961-9, 2015 Dec.

Article en En | MEDLINE | ID: mdl-26205522

Asunto(s)

Alcaligenes faecalis/enzimología; Amidohidrolasas/metabolismo; Burkholderiaceae/enzimología; Glutaratos/metabolismo; Imidas/metabolismo; Proteínas Recombinantes/metabolismo; Amidohidrolasas/química; Amidohidrolasas/genética; Amidohidrolasas/aislamiento & purificación; Estabilidad de Enzimas; Concentración de Iones de Hidrógeno; Hidrólisis; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/aislamiento & purificación; Homología de Secuencia de Aminoácido; Temperatura; Ácido gamma-Aminobutírico/metabolismo

Palabras clave

3-substituted glutarimide; Bacterial screening; Desymmetrization; GABA synthesis; Imidase; Stereoselective hydrolysis

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Alcaligenes faecalis / Burkholderiaceae / Amidohidrolasas / Glutaratos / Imidas Idioma: En Año: 2015 Tipo del documento: Article

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