3-D reconstruction and multiple marker analysis of mouse proepicardial endothelial cell population.
Microvasc Res
; 102: 54-69, 2015 Nov.
Article
en En
| MEDLINE
| ID: mdl-26277230
ABSTRACT
BACKGROUND:
The proepicardium (PE), a transient embryonic structure crucial for the development of the epicardium and heart, contains its own population of endothelial cells (ECs). The aim of our study was to determine the pattern, anatomical orientation and phenotypic marker expression of the endothelial cell network within the PE.RESULTS:
Immunohistochemical findings revealed that proepicardial ECs express both early and late EC-specific markers such as CD31, Flk-1, Lyve-1 and Tie-2 but not SCL/Tal1, vWF, Dll4 or Notch1. Proepicardial ECs are present in the vicinity of the sinus venosus (SV) and form a continuous network of vascular sprouts/tubules connected with the SV endothelium, with Ter-119-positive erythroblasts in the vascular lumina.CONCLUSIONS:
On the basis of our results, we postulate the existence of a continuous network of ECs in the PE, exhibiting connection and/or patency with the SV and forming vessels/tubules/strands. Marker expression suggests that ECs are immature and undifferentiated, which was also confirmed with a transmission electron microscopy (TEM) analysis. Our results deliver new data for a better understanding of the nature of proepicardial ECs.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pericardio
/
Células Endoteliales
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Pregnancy
Idioma:
En
Año:
2015
Tipo del documento:
Article