[Extremely low frequency electromagnetic field induces apoptosis of osteosarcoma cells via oxidative stress].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 44(3): 323-8, 2015 05.
Article
en Zh
| MEDLINE
| ID: mdl-26350014
ABSTRACT
OBJECTIVE:
To investigate the effects of extremely low frequency electromagnetic field (ELF-EMF) on human osteosarcoma cells and its mechanisms.METHODS:
Human osteosarcoma MG-63 cells were exposed to 50 Hz,1 mT ELF-EMF for 1, 2 and 3 h in vitro, with or without pretreatment by reactive oxygen species (ROS) inhibitor N acetylcysteine (NAC) or p38MAPK inhibitor SB203580. The proliferation of MG-63 cells was determined by MTT method; the apoptosis rate and ROS level in MG-63 cells were detected by flow cytometry. The expression of p38MAPK in MG-63 cells was determined by Western blotting.RESULTS:
ELF-EMF decreased the viability of MG-63 cells, inhibited cell growth, induced cell apoptosis and increased the level of ROS significantly. The apoptosis rate declined significantly after treatment with ROS inhibitor NAC or p38MAPK inhibitor SB203580. After exposure to ELF-EMF, p38MAPK in MG-63 cells was activated, and the phosphorylation level was also inhibited after treatment with NAC.CONCLUSION:
ELF-EMF can induce the apoptosis of MG-63 cells. Increased ROS and p38MAPK activation may be involved in the mechanism.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Osteosarcoma
/
Apoptosis
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Estrés Oxidativo
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Campos Electromagnéticos
Límite:
Humans
Idioma:
Zh
Año:
2015
Tipo del documento:
Article