Using a Personal Glucose Meter and Alkaline Phosphatase for Point-of-Care Quantification of Galactose-1-Phosphate Uridyltransferase in Clinical Galactosemia Diagnosis.
Chem Asian J
; 10(10): 2221-7, 2015 Oct.
Article
en En
| MEDLINE
| ID: mdl-26350570
ABSTRACT
The personal glucose meter (PGM) was recently shown to be a general meter to detect many targets. Most studies, however, focus on transforming either target binding or enzymatic activity that cleaves an artificial substrate into the production of glucose. More importantly, almost all reports exhibit their methods by using artificial samples, such as buffers or serum samples spiked with the targets. To expand the technology to even broader targets and to validate its potential in authentic, more complex clinical samples, we herein report expansion of the PGM method by using alkaline phosphatase (ALP) that links the enzymatic activity of galactose-1-phosphate uridyltransferase to the production of glucose, which allows point-of-care galactosemia diagnosis in authentic human clinical samples. Given the presence of ALP in numerous enzymatic assays for clinical diagnostics, the methods demonstrated herein advance the field closer to point-of-care detection of a wide range of targets in real clinical samples.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
UTP-Hexosa-1-Fosfato Uridililtransferasa
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Automonitorización de la Glucosa Sanguínea
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Sistemas de Atención de Punto
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Fosfatasa Alcalina
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Galactosemias
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
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Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article