The basophil surface marker CD203c identifies Aspergillus species sensitization in patients with cystic fibrosis.
J Allergy Clin Immunol
; 137(2): 436-443.e9, 2016 Feb.
Article
en En
| MEDLINE
| ID: mdl-26388311
ABSTRACT
BACKGROUND:
Colonization by Aspergillus fumigatus in patients with cystic fibrosis (CF) can cause A fumigatus sensitization and/or allergic bronchopulmonary aspergillosis (ABPA), which affects pulmonary function and clinical outcomes. Recent studies show that specific allergens upregulate the surface-expressed basophil marker CD203c in sensitized subjects, a response that can be readily measured by using flow cytometry.OBJECTIVE:
We sought to identify A fumigatus sensitization in patients with CF by using the basophil activation test (BAT).METHODS:
Patients with CF attending Beaumont Hospital were screened for study inclusion. BAT was used to identify A fumigatus sensitization. Serologic (total and A fumigatus-specific IgE), pulmonary function, and body mass index measurements were performed.RESULTS:
The BAT discriminates A fumigatus-sensitized from nonsensitized patients with CF. Persistent isolation of A fumigatus in sputum is a significant risk factor for A fumigatus sensitization. Levels of the A fumigatus-stimulated basophil activation marker CD203c inversely correlated with pulmonary function and body mass index in A fumigatus-sensitized but not nonsensitized patients with CF. Total and A fumigatus-specific IgE, but not IgG, levels are increased in A fumigatus-sensitized patients with CF and ABPA when compared with those in A fumigatus-sensitized and nonsensitized patients with CF without ABPA. Itraconazole treatment did not affect A fumigatus sensitization.CONCLUSION:
Combining the BAT with routine serologic testing allows classification of patients with CF into 3 groups nonsensitized, A fumigatus-sensitized, and ABPA. Accurate and prompt identification of A fumigatus-associated clinical status might allow early and targeted therapeutic intervention, potentially improving clinical outcomes.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pirofosfatasas
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Aspergilosis
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Aspergillus
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Basófilos
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Hidrolasas Diéster Fosfóricas
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Fibrosis Quística
Tipo de estudio:
Diagnostic_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Límite:
Female
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Humans
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Male
Idioma:
En
Año:
2016
Tipo del documento:
Article