Identification of an N-acetylglucosamine kinase essential for UDP-N-acetylglucosamine salvage synthesis in Arabidopsis.
FEBS Lett
; 589(21): 3258-62, 2015 Oct 24.
Article
en En
| MEDLINE
| ID: mdl-26408204
ABSTRACT
Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) donates GlcNAc for various glycans and glycoconjugates. We previously found that GlcNAc supplementation increases the UDP-GlcNAc content in Arabidopsis; however, the metabolic pathway was undefined. Here, we show that the homolog of human GlcNAc kinase (GNK) is conserved in land plants. Enzymatic assays of the Arabidopsis homologous protein (AtGNK) revealed kinase activity that was highly specific for GlcNAc. We also demonstrate the role of AtGNK in plants by using its knockout mutant, which presents lower UDP-GlcNAc contents and is insensitive to GlcNAc supplementation. Moreover, our results demonstrate the presence of a GlcNAc salvage pathway in plants.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Uridina Difosfato N-Acetilgalactosamina
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Arabidopsis
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Fosfotransferasas (Aceptor de Grupo Alcohol)
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Proteínas de Arabidopsis
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article