Stiffness of Hydrogels Regulates Cellular Reprogramming Efficiency Through Mesenchymal-to-Epithelial Transition and Stemness Markers.
Macromol Biosci
; 16(2): 199-206, 2016 Feb.
Article
en En
| MEDLINE
| ID: mdl-26439948
ABSTRACT
The stiffness of hydrogels has been reported to direct cell fate. Here, we found that the stiffness of hydrogels promotes the reprogramming of mouse embryonic fibroblasts into induced pluripotent stem cells (iPSCs). We prepared cell culture substrates of various stiffnesses (0.1, 1, 4, 10, and 20 kPa) using a polyacrylamide hydrogel. We found that culture on a soft hydrogel plays an important role in inducing cellular reprogramming into iPSCs via activation of mesenchymal-to-epithelial transition and enhancement of stemness marker expression. These results suggest that physical signals at the interface between cell and substrate can be used as a potent regulator to promote cell fate changes associated with reprogramming into iPSCs, which may lead to effective and reproducible iPSC-production.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Hidrogeles
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Embrión de Mamíferos
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Reprogramación Celular
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Células Madre Pluripotentes Inducidas
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Fibroblastos
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Técnicas de Reprogramación Celular
Límite:
Animals
Idioma:
En
Año:
2016
Tipo del documento:
Article