Molecular "light switch" [Ru(phen)2dppzidzo](2+) monitoring the aggregation of tau.
Analyst
; 140(22): 7513-7, 2015 Nov 21.
Article
en En
| MEDLINE
| ID: mdl-26460552
ABSTRACT
Monitoring the aggregation of the tau protein is a key protocol for elucidating the pathogenic mechanism of Alzheimer's disease. In the present article, [Ru(phen)2dppzidzo](2+), a "light switch" ruthenium(ii) complex, was presented as a new monitoring probe for the aggregation of a tau R3 peptide, the third repeat unit of the tau microtubule-binding domain. Having little impact on the aggregation process, large fixed Stokes shift and small background luminescence made the complex a better probe for monitoring the aggregation process and quantitatively detecting tau filaments compared to thioflavin S, a commonly used fluorescent dye for staining neurofibrillary tangles and monitoring tau aggregation. Furthermore, a long luminescence lifetime of this complex could also expand its potential usage in the detection of tau filaments in the presence of short-lived fluorescent backgrounds.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Compuestos Organometálicos
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Proteínas tau
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Sustancias Luminiscentes
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Enfermedad de Alzheimer
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Agregación Patológica de Proteínas
Límite:
Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article