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Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array.
Iino, Ryota; Sakakihara, Shouichi; Matsumoto, Yoshimi; Nishino, Kunihiko.
  • Iino R; Okazaki Institute for Integrative Bioscience, Institute for Molecular Science, National Institutes of Natural Sciences, Aichi, 444-8787, Japan. iino@ims.ac.jp.
  • Sakakihara S; Department of Functional Molecular Science, School of Physical Sciences, The Graduate University for Advanced Studies (SOKENDAI), Kanagawa, 240-0193, Japan. iino@ims.ac.jp.
  • Matsumoto Y; Technical Division, Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
  • Nishino K; Laboratory of Microbiology and Infectious Diseases, Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
Methods Mol Biol ; 1333: 101-9, 2016.
Article en En | MEDLINE | ID: mdl-26468103
ABSTRACT
A directly accessible femtoliter droplet array as a platform for single-cell detection and collection of persister bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Tolerancia a Medicamentos / Microtecnología / Análisis de la Célula Individual / Antibacterianos Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Año: 2016 Tipo del documento: Article
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Tolerancia a Medicamentos / Microtecnología / Análisis de la Célula Individual / Antibacterianos Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Año: 2016 Tipo del documento: Article