[Statins enhance anti-tumor effect of suberoylanilide hydroxamic acid on human non-small cell lung carcinoma cells].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 44(5): 500-5, 2015 09.
Article
en Zh
| MEDLINE
| ID: mdl-26713523
ABSTRACT
OBJECTIVE:
To evaluate the anti-tumor effect of the combination of suberoylanilide hydroxamic acid(SAHA) with statins(lovastatin or simvastatin) on non-small cell lung carcinoma(NSCLC) cells.METHODS:
Human NSCLC A549 cells were treated with SAHA in combination of lovastatin or simvastatin. The cell growth was analyzed by SRB method, and the apoptosis of A549 cells was assessed by flow cytometer. The expression of cleaved poly-ADP-ribose polymerase(cleaved-PARP) and p21 protein was analyzed by Western-blotting when A549 cells were challenged with 2.5µmol/L SAHA and 5µmol/L lovastatin.RESULTS:
Lovastatin and simvastatin synergized SAHA in the inhibition of A549 cells. SAHA induced apoptosis was also enhanced by lovastatin. Treatment with 2.5µmol/L SAHA significantly up-regulated the expression of p21 protein in 48 h, while the protein expression was reduced in combined treatment with 5µmol/L lovastatin.CONCLUSION:
Statins can synergize the anti-tumor effect of SAHA in human NSCLC cells through a p21-dependent way.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Apoptosis
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Carcinoma de Pulmón de Células no Pequeñas
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Inhibidores de Hidroximetilglutaril-CoA Reductasas
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Ácidos Hidroxámicos
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Antineoplásicos
Límite:
Humans
Idioma:
Zh
Año:
2015
Tipo del documento:
Article