Long-term storage and safe retrieval of human papillomavirus DNA using FTA elute cards.
J Virol Methods
; 229: 60-5, 2016 Mar.
Article
en En
| MEDLINE
| ID: mdl-26721377
ABSTRACT
Biobanking or collection and storage of specimens for future research purposes have become an essential tool in many fields of biomedical research and aims to provide a better understanding of disease mechanisms as well as the identification of disease-specific biomarkers that can navigate in complex diseases. In this study, we assessed the use of Flinders Technology Associates (FTA) cards as a long-term storage device for cervical specimens with suspected human papillomavirus (HPV) infections. HPV detection and genotyping results in liquid-based transport media were compared to HPV results from FTA cards. The overall agreement for the presence of any HPV infection between liquid-based medium and FTA cards stored for 1 year at ambient temperature was 100%. Reproducibility analysis of HPV detection and genotyping from FTA cards demonstrated that FTA cards are a reliable medium to store and preserve viral nucleic acids. Biobanking of cervical cells on FTA cards may provide a key resource for epidemiological and retrospective HPV studies.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Papillomaviridae
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Preservación Biológica
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Manejo de Especímenes
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Virología
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ADN Viral
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Técnicas Citológicas
Tipo de estudio:
Diagnostic_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Límite:
Adult
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Aged
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Female
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Humans
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Middle aged
Idioma:
En
Año:
2016
Tipo del documento:
Article