Refolding, purification, and preliminary structural characterization of the DNA-binding domain of the quorum sensing receptor RhlR from Pseudomonas aeruginosa.

Lixa, Carolina; Marques, Adriana F; Cortines, Juliana R; Neves, Bianca C; Oliveira, Danielle M P; Anobom, Cristiane D; Lima, Luís Maurício T R; Pinheiro, Anderson S

Lixa, Carolina; Marques, Adriana F; Cortines, Juliana R; Neves, Bianca C; Oliveira, Danielle M P; Anobom, Cristiane D; Lima, Luís Maurício T R; Pinheiro, Anderson S.

Lixa C; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, Brazil.
Marques AF; School of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro 21941-590, Brazil.
Cortines JR; Department of Virology, Institute of Microbiology Paulo de Goés, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
Neves BC; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, Brazil.
Oliveira DM; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, Brazil.
Anobom CD; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, Brazil.
Lima LM; School of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro 21941-590, Brazil.
Pinheiro AS; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, Brazil. Electronic address: pinheiro@iq.ufrj.br.

Protein Expr Purif ; 121: 31-40, 2016 May.

Article en En | MEDLINE | ID: mdl-26792557

Asunto(s)

Proteínas Bacterianas/química; Proteínas de Unión al ADN/química; Percepción de Quorum/genética; Proteínas Bacterianas/genética; Proteínas Bacterianas/aislamiento & purificación; Proteínas de Unión al ADN/genética; Proteínas de Unión al ADN/aislamiento & purificación; Escherichia coli/genética; Regulación Bacteriana de la Expresión Génica; Regiones Promotoras Genéticas; Pliegue de Proteína; Pseudomonas aeruginosa/genética

Palabras clave

Purification; Quorum sensing; Receptor; Refolding; RhlR

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Proteínas de Unión al ADN / Percepción de Quorum Idioma: En Año: 2016 Tipo del documento: Article

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