Whey protein concentrate enhances intestinal integrity and influences transforming growth factor-ß1 and mitogen-activated protein kinase signalling pathways in piglets after lipopolysaccharide challenge.
Br J Nutr
; 115(6): 984-93, 2016 Mar 28.
Article
en En
| MEDLINE
| ID: mdl-26810899
ABSTRACT
Whey protein concentrate (WPC) has been reported to have protective effects on the intestinal barrier. However, the molecular mechanisms involved are not fully elucidated. Transforming growth factor-ß1 (TGF-ß1) is an important component in the WPC, but whether TGF-ß1 plays a role in these processes is not clear. The aim of this study was to investigate the protective effects of WPC on the intestinal epithelial barrier as well as whether TGF-ß1 is involved in these protection processes in a piglet model after lipopolysaccharide (LPS) challenge. In total, eighteen weanling pigs were randomly allocated to one of the following three treatment groups (1) non-challenged control and control diet; (2) LPS-challenged control and control diet; (3) LPS+5 %WPC diet. After 19 d of feeding with control or 5 %WPC diets, pigs were injected with LPS or saline. At 4 h after injection, pigs were killed to harvest jejunal samples. The results showed that WPC improved (P<0·05) intestinal morphology, as indicated by greater villus height and villus heightcrypt depth ratio, and intestinal barrier function, which was reflected by increased transepithelial electrical resistance and decreased mucosal-to-serosal paracellular flux of dextran (4 kDa), compared with the LPS group. Moreover, WPC prevented the LPS-induced decrease (P<0·05) in claudin-1, occludin and zonula occludens-1 expressions in the jejunal mucosae. WPC also attenuated intestinal inflammation, indicated by decreased (P<0·05) mRNA expressions of TNF-α, IL-6, IL-8 and IL-1ß. Supplementation with WPC also increased (P<0·05) TGF-ß1 protein, phosphorylated-Smad2 expression and Smad4 and Smad7 mRNA expressions and decreased (P<0·05) the ratios of the phosphorylated to total c-jun N-terminal kinase (JNK) and p38 (phospho-JNKJNK and p-p38p38), whereas it increased (P<0·05) the ratio of extracellular signal-regulated kinase (ERK) (phospho-ERKERK). Collectively, these results suggest that dietary inclusion of WPC attenuates the LPS-induced intestinal injury by improving mucosal barrier function, alleviating intestinal inflammation and influencing TGF-ß1 canonical Smad and mitogen-activated protein kinase signalling pathways.
Palabras clave
EGF epidermal growth factor; ERK extracellular signal-regulated kinase; FD4 fluorescein isothiocyanate dextran 4 kDa; Intestinal integrity; JNK c-jun N-terminal kinase; LF lactoferrin; LPS lipopolysaccharide; MAPK mitogen-activated protein kinase; Mitogen-activated protein kinase; Piglets; TER transepithelial electrical resistance; TGF-ß1 transforming growth factor-ß1; Transforming growth factor-ß1; WPC whey protein concentrate; Whey protein concentrate; ZO-1 zonula occludens-1
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Suplementos Dietéticos
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Modelos Animales de Enfermedad
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Enterocolitis
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Proteínas de Uniones Estrechas
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Proteína de Suero de Leche
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Mucosa Intestinal
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Intestinos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2016
Tipo del documento:
Article