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Insights into natural products biosynthesis from analysis of 490 polyketide synthases from Fusarium.
Brown, Daren W; Proctor, Robert H.
  • Brown DW; Mycotoxin Prevention and Applied Microbiology, U.S. Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N. University St., Peoria, IL 61604, USA. Electronic address: daren.brown@ars.usda.gov.
  • Proctor RH; Mycotoxin Prevention and Applied Microbiology, U.S. Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N. University St., Peoria, IL 61604, USA.
Fungal Genet Biol ; 89: 37-51, 2016 Apr.
Article en En | MEDLINE | ID: mdl-26826610
ABSTRACT
Species of the fungus Fusarium collectively cause disease on almost all crop plants and produce numerous natural products (NPs), including some of the mycotoxins of greatest concern to agriculture. Many Fusarium NPs are derived from polyketide synthases (PKSs), large multi-domain enzymes that catalyze sequential condensation of simple carboxylic acids to form polyketides. To gain insight into the biosynthesis of polyketide-derived NPs in Fusarium, we retrieved 488 PKS gene sequences from genome sequences of 31 species of the fungus. In addition to these apparently functional PKS genes, the genomes collectively included 81 pseudogenized PKS genes. Phylogenetic analysis resolved the PKS genes into 67 clades, and based on multiple lines of evidence, we propose that homologs in each clade are responsible for synthesis of a polyketide that is distinct from those synthesized by PKSs in other clades. The presence and absence of PKS genes among the species examined indicated marked differences in distribution of PKS homologs. Comparisons of Fusarium PKS genes and genes flanking them to those from other Ascomycetes provided evidence that Fusarium has the genetic potential to synthesize multiple NPs that are the same or similar to those reported in other fungi, but that have not yet been reported in Fusarium. The results also highlight ways in which such analyses can help guide identification of novel Fusarium NPs and differences in NP biosynthetic capabilities that exist among fungi.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Productos Biológicos / Sintasas Poliquetidas / Policétidos / Fusarium / Genes Fúngicos Idioma: En Año: 2016 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Productos Biológicos / Sintasas Poliquetidas / Policétidos / Fusarium / Genes Fúngicos Idioma: En Año: 2016 Tipo del documento: Article