Structure modeling and functional analysis of recombinant dextransucrase from Weissella confusa Cab3 expressed in Lactococcus lactis.

Shukla, Shraddha; Verma, Anil Kumar; Kajala, Ilkka; Nyyssolä, Antti; Baruah, Rwivoo; Katina, Kati; Juvonen, Riikka; Tenkanen, Maija; Goyal, Arun.

Shukla S; a Department of Biosciences and Bioengineering , Indian Institute of Technology Guwahati , Guwahati , Assam , India.
Verma AK; a Department of Biosciences and Bioengineering , Indian Institute of Technology Guwahati , Guwahati , Assam , India.
Kajala I; b VTT Technical Research Centre of Finland , Espoo , Finland.
Nyyssolä A; b VTT Technical Research Centre of Finland , Espoo , Finland.
Baruah R; a Department of Biosciences and Bioengineering , Indian Institute of Technology Guwahati , Guwahati , Assam , India.
Katina K; b VTT Technical Research Centre of Finland , Espoo , Finland.
Juvonen R; c Department of Food and Environmental Sciences , University of Helsinki , Helsinki , Finland.
Tenkanen M; b VTT Technical Research Centre of Finland , Espoo , Finland.
Goyal A; c Department of Food and Environmental Sciences , University of Helsinki , Helsinki , Finland.

Prep Biochem Biotechnol ; 46(8): 822-832, 2016 Nov 16.

Article en En | MEDLINE | ID: mdl-26861959

Asunto(s)

Glucosiltransferasas/química; Weissella/enzimología; Secuencia de Aminoácidos; Dominio Catalítico; Clonación Molecular; Dextranos/metabolismo; Glucosiltransferasas/genética; Glucosiltransferasas/metabolismo; Lactococcus lactis/genética; Modelos Moleculares; Resonancia Magnética Nuclear Biomolecular; Conformación Proteica; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Alineación de Secuencia; Weissella/química; Weissella/genética; Weissella/metabolismo

Palabras clave

Dextran; Lactococcus lactis; NMR; Weissella confuse; dextransucrase; homology modeling

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