Rapid evaluation of artesunate quality with a specific monoclonal antibody-based lateral flow dipstick.
Anal Bioanal Chem
; 408(22): 6003-8, 2016 Sep.
Article
en En
| MEDLINE
| ID: mdl-26873200
ABSTRACT
Artesunate is a frontline antimalarial drug for treating Plasmodium falciparum malaria. To produce specific antibodies to artesunate, the carboxyl group of artesunate was directly conjugated to carrier protein as the immunogen. A specific monoclonal antibody (mAb) 3D82G6 against artesunate was obtained by high-throughput screening of positive hybridoma clones. This monoclonal antibody had 4.0, 0.5, and 0.9 % cross reactivities with artemisinin, dihydroartemisinin, and artemether, respectively. A dipstick immunoassay was developed, and the indicator range for artesunate was 1000-2000 ng mL(-1). No interference was observed with artemisinin, dihydroartemisinin, artemether, and other commonly used antimalarial drugs for up to 20,000 ng mL(-1). The dipsticks were used for determination of artesunate contents in commercial drugs, and the results were agreeable with those determined by high-performance liquid chromatography. This dipstick, with its specificity and sensitivity for artesunate and simplicity to use, makes it a potential point-of-care device for rapid quality evaluation of artesunate-containing antimalarial drugs. Graphical Abstract Specific monoclonal antibody-based lateral flow dipstick for artesunate.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Inmunoensayo
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Sistemas de Atención de Punto
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Artemisininas
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Anticuerpos Inmovilizados
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Anticuerpos Monoclonales
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Antimaláricos
Tipo de estudio:
Evaluation_studies
Límite:
Animals
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Humans
Idioma:
En
Año:
2016
Tipo del documento:
Article