Lymphotoxin cDNA clones from a HTLV-I-carrying T cell line HUT-102.
AIDS Res Hum Retroviruses
; 5(6): 663-70, 1989 Dec.
Article
en En
| MEDLINE
| ID: mdl-2692660
ABSTRACT
The conditioned medium of a HTLV-I-carrying T cell line HUT-102 showed cytotoxic activity against a mouse fibroblast cell line L-M. We prepared the cDNA library from HUT-102 poly(A)+ RNA and screened it using oligonucleotide probes that correspond to the amino acid sequences conserved in tumor necrosis factor (TNF) and lymphotoxin (LT). As a result we obtained two kinds of cDNA clones encoding LT in which amino acid residue 26t of mature LT is different; one is Asn and another is Thr. The sequence of the genomic clones obtained using a polymerase chain reaction method showed that the HUT-102 genome also contains two types of LT genes. Recombinant LTs expressed in Escherichia coli exhibited the same level of cytolytic activity against L-M cells. These results indicate that the cytotoxin constitutively produced by HUT-102 cells include two kinds of LT.
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Banco de datos:
MEDLINE
Asunto principal:
ADN
/
Virus Linfotrópico T Tipo 1 Humano
/
Linfotoxina-alfa
Límite:
Humans
Idioma:
En
Año:
1989
Tipo del documento:
Article