Neurotropism of Saffold virus in a mouse model.
J Gen Virol
; 97(6): 1350-1355, 2016 06.
Article
en En
| MEDLINE
| ID: mdl-26959376
ABSTRACT
Saffold virus (SAFV) is a highly seroprevalent human Cardiovirus discovered recently. No clear association between SAFV infection and human disease has been established. Rare infection cases, however, correlated with neurological symptoms. To gain insight into the pathogenesis potential of the virus, we performed experimental mouse infection with SAFV strains of genotypes 2 and 3 (SAFV-2 and SAFV-3). After intraperitoneal infection, both strains exhibited a typical Cardiovirus tropism. Viral load was most prominent in the pancreas. Heart, spleen, brain and spinal cord were also infected. In IFN-receptor 1 deficient (IFNAR-KO) mice, SAFV-3 caused a severe encephalitis. The virus was detected by immunohistochemistry in many parts of the brain and spinal cord, both in neurons and astrocytes, but astrocyte infection was more extensive. In vitro, SAFV-3 also infected astrocytes better than neurons in mixed primary cultures. Astrocytes were, however, very efficiently protected by IFN-α/ß treatment.
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Banco de datos:
MEDLINE
Asunto principal:
Theilovirus
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Infecciones por Cardiovirus
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Tropismo Viral
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Sistema Nervioso
Límite:
Animals
Idioma:
En
Año:
2016
Tipo del documento:
Article