Comparison of Different Matrices as Potential Quality Control Samples for Neurochemical Dementia Diagnostics.
J Alzheimers Dis
; 52(1): 51-64, 2016 03 01.
Article
en En
| MEDLINE
| ID: mdl-26967210
ABSTRACT
BACKGROUND:
Assay-vendor independent quality control (QC) samples for neurochemical dementia diagnostics (NDD) biomarkers are so far commercially unavailable. This requires that NDD laboratories prepare their own QC samples, for example by pooling leftover cerebrospinal fluid (CSF) samples.OBJECTIVE:
To prepare and test alternative matrices for QC samples that could facilitate intra- and inter-laboratory QC of the NDD biomarkers.METHODS:
Three matrices were validated in this study (A) human pooled CSF, (B) Aß peptides spiked into human prediluted plasma, and (C) Aß peptides spiked into solution of bovine serum albumin in phosphate-buffered saline. All matrices were tested also after supplementation with an antibacterial agent (sodium azide). We analyzed short- and long-term stability of the biomarkers with ELISA and chemiluminescence (Fujirebio Europe, MSD, IBL International), and performed an inter-laboratory variability study.RESULTS:
NDD biomarkers turned out to be stable in almost all samples stored at the tested conditions for up to 14 days as well as in samples stored deep-frozen (at - 80°C) for up to one year. Sodium azide did not influence biomarker stability. Inter-center variability of the samples sent at room temperature (pooled CSF, freeze-dried CSF, and four artificial matrices) was comparable to the results obtained on deep-frozen samples in other large-scale projects.CONCLUSION:
Our results suggest that it is possible to replace self-made, CSF-based QC samples with large-scale volumes of QC materials prepared with artificial peptides and matrices. This would greatly facilitate intra- and inter-laboratory QC schedules for NDD measurements.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pruebas de Química Clínica
/
Demencia
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Año:
2016
Tipo del documento:
Article