A method for culturing Gram-negative skin microbiota.
BMC Microbiol
; 16: 60, 2016 Apr 06.
Article
en En
| MEDLINE
| ID: mdl-27052736
ABSTRACT
BACKGROUND:
Commensal Gram-negative (CGN) microbiota have been identified on human skin by DNA sequencing; however, methods to reliably culture viable Gram-negative skin organisms have not been previously described.RESULTS:
Through the use of selective antibiotics and minimal media we developed methods to culture CGN from skin swabs. We identified several previously uncharacterized CGN at the species level by optimizing growth conditions and limiting the inhibitory effects of nutrient shock, temperature, and bacterial competition, factors that may have previously limited CGN isolation from skin cultures.CONCLUSIONS:
Our protocol will permit future functional studies on the influences of CGN on skin homeostasis and disease.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Piel
/
Técnicas Bacteriológicas
/
Bacterias Gramnegativas
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
2016
Tipo del documento:
Article