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Hepatitis B plasmonic biosensor for the analysis of clinical serum samples.
Riedel, Tomás; Surman, Frantisek; Hageneder, Simone; Pop-Georgievski, Ognen; Noehammer, Christa; Hofner, Manuela; Brynda, Eduard; Rodriguez-Emmenegger, Cesar; Dostálek, Jakub.
  • Riedel T; Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: riedel@imc.cas.cz.
  • Surman F; Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
  • Hageneder S; Biosensor Technologies, AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria.
  • Pop-Georgievski O; Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
  • Noehammer C; Molecular Diagnostics, Health and Environment Dpt., AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria.
  • Hofner M; Molecular Diagnostics, Health and Environment Dpt., AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria.
  • Brynda E; Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
  • Rodriguez-Emmenegger C; Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic; DWI - Leibniz Institute for Interactive Materials and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Forckenbeckstraße 50, 52074 Aachen, Germany. Electronic address: ro
  • Dostálek J; Biosensor Technologies, AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria. Electronic address: jakub.dostalek@ait.ac.at.
Biosens Bioelectron ; 85: 272-279, 2016 Nov 15.
Article en En | MEDLINE | ID: mdl-27179568
ABSTRACT
A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Virus de la Hepatitis B / Resonancia por Plasmón de Superficie / Hepatitis B / Anticuerpos Antivirales Límite: Humans Idioma: En Año: 2016 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Virus de la Hepatitis B / Resonancia por Plasmón de Superficie / Hepatitis B / Anticuerpos Antivirales Límite: Humans Idioma: En Año: 2016 Tipo del documento: Article