Electrochemiluminescence (ECL) immunosensor for detection of Francisella tularensis on screen-printed gold electrode array.
Anal Bioanal Chem
; 408(25): 7147-53, 2016 Oct.
Article
en En
| MEDLINE
| ID: mdl-27255102
ABSTRACT
An electrochemiluminescence (ECL) immunosensor for the rapid detection of the Francisella tularensis pathogen using whole antibodies or antibody fragments as capture biomolecule is described. A sandwich immunoassay was used with either lipopolysaccharide (LPS) or the whole inactivated bacterial cell (LVS) as a target, while Ru(bpy)3 (2+)-encapsulated silicate nanoparticles were linked to the secondary antibody and used as ECL labels. The assay was performed in a fluidic chip housed in a custom-built black box incorporating electronics, optics and fluidics. The obtained limit of detection for LPS was 0.4 ng/mL, while for the LVS it was 70 and 45 bacteria/mL when the capturing molecule was the whole antibody and the antibody F(ab) fragment, respectively.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Inmunoensayo
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Anticuerpos Inmovilizados
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Técnicas Electroquímicas
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Francisella tularensis
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Oro
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Mediciones Luminiscentes
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Anticuerpos Antibacterianos
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
Límite:
Humans
Idioma:
En
Año:
2016
Tipo del documento:
Article