Determination of the inhibitor dissociation constant of an individual unmodified enzyme molecule in free solution.

Crawford, Jeremie J; Hollett, Joshua W; Craig, Douglas B

Crawford, Jeremie J; Hollett, Joshua W; Craig, Douglas B.

Crawford JJ; Chemistry Department, University of Manitoba, Winnipeg, Canada.
Hollett JW; Chemistry Department, University of Winnipeg, Winnipeg, Canada.
Craig DB; Chemistry Department, University of Winnipeg, Winnipeg, Canada.

Electrophoresis ; 37(15-16): 2217-25, 2016 08.

Article en En | MEDLINE | ID: mdl-27271375

Asunto(s)

Unión Competitiva; Pruebas de Enzimas/métodos; Escherichia coli/enzimología; Escherichia coli/metabolismo; Electroforesis Capilar/métodos; Cinética; Modelos Químicos; Reproducibilidad de los Resultados; Ribosa; beta-Galactosidasa/metabolismo

Palabras clave

Heterogeneity KI, Km; Single molecule; ß-galactosidase

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Unión Competitiva / Escherichia coli / Pruebas de Enzimas Tipo de estudio: Prognostic_studies Idioma: En Año: 2016 Tipo del documento: Article

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