Recombinant measles virus incorporating heterologous viral membrane proteins for use as vaccines.
J Gen Virol
; 97(9): 2117-2128, 2016 09.
Article
en En
| MEDLINE
| ID: mdl-27311834
ABSTRACT
Recombinant measles virus (rMV) vectors expressing heterologous viral membrane protein antigens are potentially useful as vaccines. Genes encoding the mumps virus haemagglutinin-neuraminidase (MuV-HN), the influenza virus haemagglutinin (Flu-HA) or the respiratory syncytial virus fusion (RSV-F) proteins were inserted into the genome of a live attenuated vaccine strain of measles virus. Additionally, in this case rMV with the MuV-HN or the influenza HA inserts, chimeric constructs were created that harboured the measles virus native haemagglutinin or fusion protein cytoplasmic domains. In all three cases, sucrose-gradient purified preparations of rMV were found to have incorporated the heterologous viral membrane protein on the viral membrane. The possible utility of rMV expressing RSV-F (rMV.RSV-F) as a vaccine was tested in a cotton rat challenge model. Vaccination with rMV.RSV-F efficiently induced neutralizing antibodies against RSV and protected animals from infection with RSV in the lungs.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Virus Sincitiales Respiratorios
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Portadores de Fármacos
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Vacunas Virales
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Proteínas de la Matriz Viral
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Infecciones por Virus Sincitial Respiratorio
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Vectores Genéticos
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Virus del Sarampión
Límite:
Animals
Idioma:
En
Año:
2016
Tipo del documento:
Article