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MAL and TMEM220 are novel DNA methylation markers in human gastric cancer.
Choi, Boram; Han, Tae-Su; Min, Jimin; Hur, Keun; Lee, Sun-Min; Lee, Hyuk-Joon; Kim, Young-Joon; Yang, Han-Kwang.
  • Choi B; a Cancer Research Institute , Seoul National University College of Medicine , Seoul , Korea.
  • Han TS; b Division of Genetics , Cancer Research Institute, Kanazawa University , Kanazawa , Japan.
  • Min J; c Department of Biochemistry and Cell Biology , Cell and Matrix Research Institute, School of Medicine, Kyungpook National University , Daegu , Korea.
  • Hur K; c Department of Biochemistry and Cell Biology , Cell and Matrix Research Institute, School of Medicine, Kyungpook National University , Daegu , Korea.
  • Lee SM; d Department of Biochemistry, College of Life Science and Technology , Yonsei University , Seoul , Korea.
  • Lee HJ; a Cancer Research Institute , Seoul National University College of Medicine , Seoul , Korea.
  • Kim YJ; e Department of Integrated OMICS for Biomedical Science Graduate School , Yonsei University , Seoul , Korea.
  • Yang HK; e Department of Integrated OMICS for Biomedical Science Graduate School , Yonsei University , Seoul , Korea.
Biomarkers ; 22(1): 35-44, 2017 Feb.
Article en En | MEDLINE | ID: mdl-27329150
ABSTRACT
CONTEXT Gastric cancer (GC) is the fourth most common cause of cancer-related deaths worldwide.

OBJECTIVE:

To determine the mRNA-expression of the MAL, TMEM220, MMP28, IL-19 and HOPX genes and analyse the methylation statuses of MAL and TMEM220. MATERIALS AND

METHODS:

Gene-expression levels were analysed in 10 GC cell lines and 30 matched pairs of GC and normal mucosa (NM) gastric tissue specimens in real-time reverse-transcriptase polymerase chain reactions. Gene methylation was evaluated by bisulphite sequencing. Detailed gene-methylation patterns were confirmed by pyrosequencing analysis.

RESULTS:

MAL, TMEM220, MMP28 and IL-19 were significantly down-regulated in GC cell lines and GC tissues compared to NM tissues. MAL and TMEM220 were highly methylated in GC tissues, and methylation inversely correlated with expression. MAL and TMEM220 expression were restored by treatment with 5-aza-2'-deoxycytidine. MAL and TMEM220 were specifically methylated and were down-regulated in human GC. DISCUSSION AND

CONCLUSION:

These loci may serve as novel methylation markers for patients with GC.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Biomarcadores de Tumor / Regulación Neoplásica de la Expresión Génica / Metilación de ADN / Proteínas Proteolipídicas Asociadas a Mielina y Linfocito / Proteínas de la Membrana Límite: Humans Idioma: En Año: 2017 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Biomarcadores de Tumor / Regulación Neoplásica de la Expresión Génica / Metilación de ADN / Proteínas Proteolipídicas Asociadas a Mielina y Linfocito / Proteínas de la Membrana Límite: Humans Idioma: En Año: 2017 Tipo del documento: Article