A method for amplification of unknown flanking sequences based on touchdown PCR and suppression-PCR.

Gao, Song; He, Dan; Li, Guangquan; Zhang, Yanhua; Lv, Huiying; Wang, Li

Gao, Song; He, Dan; Li, Guangquan; Zhang, Yanhua; Lv, Huiying; Wang, Li.

Gao S; Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China. Electronic address: gaosong850118@me.com.
He D; Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China. Electronic address: hedan@jlu.edu.cn.
Li G; Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China; Jilin Provincial Key Laboratory on Molecular and Chemical Genetic, Second Hospital of Jilin
Zhang Y; Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China; Department of Biology, Plant Science College of Jilin University, Changchun, 130062, China.
Lv H; Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China; Science Research Certer, China-Japan Union Hospital, Jilin University, Changchun, 130021, C
Wang L; Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China. Electronic address: wli99@jlu.edu.cn.

Anal Biochem ; 509: 79-81, 2016 09 15.

Article en En | MEDLINE | ID: mdl-27393656

Asunto(s)

Agrobacterium tumefaciens/química; Aspergillus fumigatus/química; ADN Bacteriano/química; ADN de Hongos/química; Reacción en Cadena de la Polimerasa/métodos; Sporothrix/química; Agrobacterium tumefaciens/genética; Aspergillus fumigatus/genética; ADN Bacteriano/genética; ADN de Hongos/genética; Sporothrix/genética

Palabras clave

Agrobacterium tumefaciens-Mediated transformation; Flanking sequences; Suppression-PCR; Thermal asymmetric staggered PCR (TAIL-PCR); Touchdown TAIL-PCR (TD-TAIL PCR)

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Aspergillus fumigatus / Sporothrix / ADN Bacteriano / ADN de Hongos / Reacción en Cadena de la Polimerasa / Agrobacterium tumefaciens Idioma: En Año: 2016 Tipo del documento: Article

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