Radiation Dose Estimation by Automated Cytogenetic Biodosimetry.
Radiat Prot Dosimetry
; 172(1-3): 207-217, 2016 Dec.
Article
en En
| MEDLINE
| ID: mdl-27412514
ABSTRACT
The dose from ionizing radiation exposure can be interpolated from a calibration curve fit to the frequency of dicentric chromosomes (DCs) at multiple doses. As DC counts are manually determined, there is an acute need for accurate, fully automated biodosimetry calibration curve generation and analysis of exposed samples. Software, the Automated Dicentric Chromosome Identifier (ADCI), is presented which detects and discriminates DCs from monocentric chromosomes, computes biodosimetry calibration curves and estimates radiation dose. Images of metaphase cells from samples, exposed at 1.4-3.4 Gy, that had been manually scored by two reference laboratories were reanalyzed with ADCI. This resulted in estimated exposures within 0.4-1.1 Gy of the physical dose. Therefore, ADCI can determine radiation dose with accuracies comparable to standard triage biodosimetry. Calibration curves were generated from metaphase images in ~10 h, and dose estimations required ~0.8 h per 500 image sample. Running multiple instances of ADCI may be an effective response to a mass casualty radiation event.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Radiometría
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Bioensayo
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Robótica
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Interfaz Usuario-Computador
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Programas Informáticos
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Interpretación de Imagen Asistida por Computador
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Aberraciones Cromosómicas
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
2016
Tipo del documento:
Article