Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions.
Colloids Surf B Biointerfaces
; 147: 36-44, 2016 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-27478961
ABSTRACT
Amyloid fibrils have recently gained a lot of attention due to their morphology, functionality and mechanical strength, allowing for their application in nanofiber-based materials, biosensors, bioactive membranes and tissue engineering scaffolds. The in vitro production of amyloid fibrils is still a slow process, thus hampering the massive production of nanofibers and its consequent use. This work presents a new and faster (2-3h) fibrillation method for hen egg white lysozyme (HEWL) using a deep eutectic solvent based on cholinium chloride and acetic acid. Nanofibers with dimensions of 0.5-1µm in length and 0.02-0.1µm in thickness were obtained. Experimental variables such as temperature and pH were also studied, unveiling their influence in fibrillation time and nanofibers morphology. These results open a new scope for protein fibrillation into nanofibers with applications ranging from medicine to soft matter and nanotechnology.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Solventes
/
Muramidasa
/
Andamios del Tejido
/
Nanofibras
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2016
Tipo del documento:
Article