Field Effect Microparticle Generation for Cell Microencapsulation.
Methods Mol Biol
; 1479: 57-70, 2017.
Article
en En
| MEDLINE
| ID: mdl-27738926
ABSTRACT
The diameter and sphericity of alginate-poly-L-lysine-alginate microcapsules, determined by the size and the shape of calcium alginate microspheres, affect their in vivo durability and biocompatibility and the results of transplantation. The commonly used air-jet spray method generates microspheres with a wider variation in diameter, larger sphere morphology, and evenly distributed encapsulated cells. In order to overcome these drawbacks, we designed a field effect microparticle generator to create a stable electric field to prepare microparticles with a smaller diameter and more uniform morphology. Using this electric field microparticle generator the encapsulated cells will be located at the periphery of the microspheres, and thus the supply of oxygen and nutrients for the encapsulated cells will be improved compared with the centrally located encapsulated cells in the air-jet spray method.
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Banco de datos:
MEDLINE
Asunto principal:
Polilisina
/
Trasplante de Islotes Pancreáticos
/
Islotes Pancreáticos
/
Células Inmovilizadas
/
Composición de Medicamentos
/
Alginatos
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Año:
2017
Tipo del documento:
Article