Efficient biosynthesis of ethyl (R)-4-chloro-3-hydroxybutyrate using a stereoselective carbonyl reductase from Burkholderia gladioli.

Chen, Xiang; Liu, Zhi-Qiang; Lin, Chao-Ping; Zheng, Yu-Guo

Chen, Xiang; Liu, Zhi-Qiang; Lin, Chao-Ping; Zheng, Yu-Guo.

Chen X; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Liu ZQ; Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China.
Lin CP; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Zheng YG; Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China.

BMC Biotechnol ; 16(1): 70, 2016 Oct 18.

Article en En | MEDLINE | ID: mdl-27756363

Asunto(s)

Oxidorreductasas de Alcohol/metabolismo; Burkholderia gladioli/enzimología; Butiratos/metabolismo; Escherichia coli/metabolismo; Mejoramiento Genético/métodos; Ingeniería de Proteínas/métodos; Oxidorreductasas de Alcohol/química; Oxidorreductasas de Alcohol/genética; Sitios de Unión; Burkholderia gladioli/genética; Butiratos/aislamiento & purificación; Catálisis; Activación Enzimática; Estabilidad de Enzimas; Escherichia coli/genética; Unión Proteica; Estereoisomerismo

Palabras clave

Burkholderia gladioli; Carbonyl reductases; Co-expression; Ethyl (R)-4-chloro-3-hydroxybutyrate; Ethyl 4-chloro-3-oxobutanoate

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Butiratos / Ingeniería de Proteínas / Mejoramiento Genético / Burkholderia gladioli / Oxidorreductasas de Alcohol / Escherichia coli Idioma: En Año: 2016 Tipo del documento: Article

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