Glutamate-induced RNA localization and translation in neurons.
Proc Natl Acad Sci U S A
; 113(44): E6877-E6886, 2016 11 01.
Article
en En
| MEDLINE
| ID: mdl-27791158
ABSTRACT
Localization of mRNA is required for protein synthesis to occur within discrete intracellular compartments. Neurons represent an ideal system for studying the precision of mRNA trafficking because of their polarized structure and the need for synapse-specific targeting. To investigate this targeting, we derived a quantitative and analytical approach. Dendritic spines were stimulated by glutamate uncaging at a diffraction-limited spot, and the localization of single ß-actin mRNAs was measured in space and time. Localization required NMDA receptor activity, a dynamic actin cytoskeleton, and the transacting RNA-binding protein, Zipcode-binding protein 1 (ZBP1). The ability of the mRNA to direct newly synthesized proteins to the site of localization was evaluated using a Halo-actin reporter so that RNA and protein were detected simultaneously. Newly synthesized Halo-actin was enriched at the site of stimulation, required NMDA receptor activity, and localized preferentially at the periphery of spines. This work demonstrates that synaptic activity can induce mRNA localization and local translation of ß-actin where the new actin participates in stabilizing the expanding synapse in dendritic spines.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ácido Glutámico
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Transporte de ARN
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Neuronas
Límite:
Animals
Idioma:
En
Año:
2016
Tipo del documento:
Article