Inflammation enhances the vaccination potential of CD40-activated B cells in mice.
Eur J Immunol
; 47(2): 269-279, 2017 02.
Article
en En
| MEDLINE
| ID: mdl-27873323
ABSTRACT
Vaccination with antigen-pulsed CD40-activated B (CD40-B) cells can efficiently lead to the in vivo differentiation of naive CD8+ T cells into fully functional effectors. In contrast to bone marrow-derived dendritic cell (BMDC) vaccination, CD40-B cell priming does not allow for memory CD8+ T-cell generation but the reason for this deficiency is unknown. Here, we show that compared to BMDCs, murine CD40-B cells induce lower expression of several genes regulated by T-cell receptor signaling, costimulation, and inflammation (signals 1-3) in mouse T cells. The reduced provision of signals 1 and 2 by CD40-B cells can be explained by a reduction in the quality and duration of the interactions with naive CD8+ T cells as compared to BMDCs. Furthermore, CD40-B cells produce less inflammatory mediators, such as IL-12 and type I interferon, and increasing inflammation by coadministration of polyriboinosinic-polyribocytidylic acid with CD40-B-cell immunization allowed for the generation of long-lived and functional CD8+ memory T cells. In conclusion, it is possible to manipulate CD40-B-cell vaccination to promote the formation of long-lived functional CD8+ memory T cells, a key step before translating the use of CD40-B cells for therapeutic vaccination.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Polinucleótidos
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Células de la Médula Ósea
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Linfocitos B
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Linfocitos T CD8-positivos
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Inflamación
Límite:
Animals
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Humans
Idioma:
En
Año:
2017
Tipo del documento:
Article