Characterization of a secreted cytoactive factor from Trichomonas vaginalis.
Am J Trop Med Hyg
; 41(1): 18-28, 1989 Jul.
Article
en En
| MEDLINE
| ID: mdl-2788372
ABSTRACT
Trichomonas vaginalis, grown in Dulbecco's modified Eagle's medium with or without serum, produced a factor (TVF) which altered the morphology of certain mammalian cells in vitro. TVF had a Mr of approximately 250 kDa by gel filtration, approximately 50 kDa by SDS-PAGE, and was heat (56 degrees C, 30 min) and pH (greater than 6 or less than 8) labile. Co-incubation of TVF with adherent target cells caused a marked rounding and clumping of BHK-21 or CHO-K1 cells, but had no effect on RK-13 or WEHI-3 cells. These morphologic changes were concentration, time, and energy dependent. Reversibility was attained by exogenous serum addition (greater than 10%) or TVF washout. Target cell perturbations were not accompanied by significant changes in growth (as measured by nuclei counts, DNA content, or 3H-thymidine incorporation), in cell leakage (as assessed by lactate dehydrogenase release), or in cell viability (by trypan blue dye exclusion). TVF-induced effects were independent of cyclic AMP and cyclic GMP levels in BHK cells exposed for 5 min-24 hr.
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Banco de datos:
MEDLINE
Asunto principal:
Trichomonas vaginalis
/
Factores Biológicos
Límite:
Animals
Idioma:
En
Año:
1989
Tipo del documento:
Article