Heterogeneity of granules of murine cytolytic T lymphocytes. Isolation of a homogeneous population of dense granules.
J Immunol Methods
; 124(1): 7-15, 1989 Nov 13.
Article
en En
| MEDLINE
| ID: mdl-2809230
ABSTRACT
A separation procedure based on Percoll gradient centrifugation is described here that allows enrichment for two main populations of granules, with average densities of 1.05 and 1.1 g/ml. The heavy-density granules, which comprise 5-20% of the total amount of membrane-lytic activity, are morphologically and biochemically more homogeneous than the light-density granules, which are contaminated with other subcellular organelles. A trypsin-like serine esterase activity, used in the past as a granule marker, was mainly found in the free cytosol and in the light-density fractions, while a small portion of this activity was associated with the heavy-density fractions. This enzyme marker is therefore not exclusively associated with granules and should not be used as the only granule marker.
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Banco de datos:
MEDLINE
Asunto principal:
Fracciones Subcelulares
/
Glicoproteínas de Membrana
/
Linfocitos T
/
Fraccionamiento Celular
/
Esterasas
Límite:
Animals
Idioma:
En
Año:
1989
Tipo del documento:
Article