Enhancement of single guide RNA transcription for efficient CRISPR/Cas-based genomic engineering.

Ui-Tei, Kumiko; Maruyama, Shohei; Nakano, Yuko

Ui-Tei, Kumiko; Maruyama, Shohei; Nakano, Yuko.

Ui-Tei K; a Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba-ken 277-8651, Japan.
Maruyama S; b Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba-ken 277-8561, Japan.
Nakano Y; c Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Genome ; 60(6): 537-545, 2017 Jun.

Article en En | MEDLINE | ID: mdl-28177825

Asunto(s)

Sistemas CRISPR-Cas/genética; Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas/genética; Genoma/genética; ARN Guía de Kinetoplastida/genética; ARN/genética; Transcripción Genética/genética; Línea Celular; ADN/genética; ARN Polimerasas Dirigidas por ADN/genética; Ingeniería Genética/métodos; Genómica/métodos; Células HEK293; Humanos

Palabras clave

ARN polymérase III; CRISPR; Cas9; RNA interference; RNA polymerase III; interférence à l'ARN; sgRNA

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Transcripción Genética / ARN / Genoma / ARN Guía de Kinetoplastida / Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas / Sistemas CRISPR-Cas Límite: Humans Idioma: En Año: 2017 Tipo del documento: Article

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