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Characterization of Fc gamma receptor IIb expression within abdominal aortic aneurysm.
Shi, Yi; Yang, Ci-Qiu; Wang, Si-Wen; Li, Wen; Li, Jie; Wang, Shen-Ming.
  • Shi Y; Division of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, China.
  • Yang CQ; Department of Breast Cancer, Cancer Center, Guangdong General Hospital, Guangdong Academy of Medical Sciences, China.
  • Wang SW; Division of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, China.
  • Li W; Laboratory of General Surgery, The First Affiliated Hospital of Sun Yat-sen University, China; Guangdong Engineering Laboratory for Diagnosis and Treatment of Vascular Disease, China.
  • Li J; Division of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, China.
  • Wang SM; Division of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, China; Guangdong Engineering Laboratory for Diagnosis and Treatment of Vascular Disease, China; Vascular Surgical Disease Research Center of Guangdong Province, China. Electronic address: shenmingwang@hotmail.com.
Biochem Biophys Res Commun ; 485(2): 295-300, 2017 04 01.
Article en En | MEDLINE | ID: mdl-28223220
ABSTRACT

OBJECTIVE:

To evaluate protein profiles and Fc gamma receptor IIb (FCGRIIB) expression in abdominal aortic aneurysm (AAA) compared to the expression in normal aortas.

METHODS:

We performed a protein array to study the protein expression profiles within AAA and normal aortic tissues. FCGRIIB was found to be significantly elevated in AAA samples. Quantitative PCR and Western blot analyses were performed to study the expression of FCGRIIB in AAA compared to that in normal aortic tissue. Immunohistochemistry was used to locate FCGRIIB and the B cell marker CD19 in AAA and normal aortas specimens.

RESULTS:

FCGRIIB was significantly elevated in AAA tissues in both mRNA (AAA vs. normal control about 5.8 folds, p < 0.001) and protein levels (AAA vs. normal control about 6.3 folds, p < 0.001). In AAA specimens, immunohistochemistry revealed that FCGRIIB localized to the area of inflammatory infiltrates, which consisted of CD 19+ B cells and other inflammatory cells. FCGRIIB and CD19 were undetectable in normal aortas.

CONCLUSIONS:

FCGRIIB was significantly elevated in AAA tissues compared to normal aortas. FCGRIIB may be involved in the pathogenesis of AAA by regulation of inflammatory reactions.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Aorta Abdominal / Receptores de IgG / Aneurisma de la Aorta Abdominal Límite: Aged / Female / Humans / Male Idioma: En Año: 2017 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Aorta Abdominal / Receptores de IgG / Aneurisma de la Aorta Abdominal Límite: Aged / Female / Humans / Male Idioma: En Año: 2017 Tipo del documento: Article