Stepwise unfolding supports a subunit model for vertebrate kinetochores.
Proc Natl Acad Sci U S A
; 114(12): 3133-3138, 2017 03 21.
Article
en En
| MEDLINE
| ID: mdl-28265097
ABSTRACT
During cell division, interactions between microtubules and chromosomes are mediated by the kinetochore, a proteinaceous structure located at the primary constriction of chromosomes. In addition to the centromere histone centromere protein A (CENP-A), 15 other members of the constitutive centromere associated network (CCAN) participate in the formation of a chromatin-associated scaffold that supports kinetochore structure. We performed a targeted screen analyzing unfolded centrochromatin from CENP-depleted chromosomes. Our results revealed that CENP-C and CENP-S are critical for the stable folding of mitotic kinetochore chromatin. Multipeak fitting algorithms revealed the presence of an organized pattern of centrochromatin packing consistent with arrangement of CENP-A-containing nucleosomes into up to five chromatin "subunits"-each containing roughly 20-30 nucleosomes. These subunits could be either layers of a boustrophedon or small loops of centromeric chromatin.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Conformación Proteica
/
Modelos Moleculares
/
Cinetocoros
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Subunidades de Proteína
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Desplegamiento Proteico
Límite:
Animals
Idioma:
En
Año:
2017
Tipo del documento:
Article