Structural analysis and enhanced production of fusaricidin from Paenibacillus kribbensis CU01 isolated from yellow loess.
J Basic Microbiol
; 57(6): 525-535, 2017 Jun.
Article
en En
| MEDLINE
| ID: mdl-28295433
ABSTRACT
A bacterial strain showing strong antifungal activity was isolated from yellow loess and was identified as Paenibacillus kribbensis CU01. Insoluble mucoidal polymers were separated from M9 culture medium via low-speed centrifugation. Most antifungal activity was associated with substances in the insoluble precipitate, which was purified by reverse phase high performance liquid chromatography. Purified fractions were analyzed using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. Two major ion peaks with mass-to-charge ratio values (m/z) at 883.6 and 897.6 were revealed. After alkaline hydrolysis and sequence analysis, two cyclic depsipeptides were identified as, fusaricidin A and fusaricidin B. Their production was significantly increased by the addition of glucose, Fe2+ , and Mn2+ to M9 medium. Maximum concentrations of produced fusaricidin A and fusaricidin B at flask-scale comprised 460 mg L-1 and 118 mg L-1 , respectively the highest production concentrations yet reported in the literature. This demonstrates that P. kribbensis CU01 has enormous commercial potential for the mass production of fusaricidin.
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Banco de datos:
MEDLINE
Asunto principal:
Microbiología del Suelo
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Proteínas Bacterianas
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Depsipéptidos
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Paenibacillus
Idioma:
En
Año:
2017
Tipo del documento:
Article